Computational approach of protein analysis using affinity chromatography: application to proteomics
نویسنده
چکیده
Computerization is necessary to build the database of chromatography coupled mass spectrometric analytical data. On the basis of this proteomic data we can identify the proteins as biomarkers which are expressing dissimilarity between healthy and disease condition. Affinity chromatography is one of the fastest liquid chromatographic methods for the separation and purification of biomolecules due to its high molecular specificity. Proteomics and the tools used to identify and understand the biochemistry of proteins and pathways are in a new stage of development and evaluation. This article briefly reviews the computation of chromatography coupled mass spectrometric data and highlighting the role of affinity chromatography
منابع مشابه
Protein-Protein Interaction Analysis of Common Top Genes in Obsessive-Compulsive disorder (OCD) and Schizophrenia: Towards New Drug Approach
Comorbidty is common among psychiatric disorders including obsessive-compulsive disorder and schizophrenia with a high rate. Many studies suggested that the disorders may have same etiological bases. In this regard, shared pathways of glutamate, dopaminergic, and serotonin are the known ones. Here, the common significant genes are examined to understand the possible molecular origin of the diso...
متن کاملProbabilistic assembly of human protein interaction networks from label-free quantitative proteomics.
Large-scale affinity purification and mass spectrometry studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes. However, the development of such networks for human proteins has been slowed by the high cost and significant technical challenges associated with systematic studies of protein interactions. To address this c...
متن کاملComputational and informatics strategies for identification of specific protein interaction partners in affinity purification mass spectrometry experiments.
Analysis of protein interaction networks and protein complexes using affinity purification and mass spectrometry (AP/MS) is among most commonly used and successful applications of proteomics technologies. One of the foremost challenges of AP/MS data is a large number of false-positive protein interactions present in unfiltered data sets. Here we review computational and informatics strategies f...
متن کاملIdentification of Agents with Potential Leishmania Malate Dehydrogenase Inhibitor Activity: A Proteomic and Molecular Docking Approach
Background and purpose: Leishmaniasis is one of the most important infectious diseases caused by different species of the Leishmania, which is a public health problem worldwide. So far, no effective vaccine is introduced for this disease and drug therapy is associated with many side effects. Therefore, this study was designed to identify novel FDA-approved compounds with anti-leishmanial activ...
متن کاملProtein-Protein Interaction Analysis of Common Top Genes in Obsessive-Compulsive disorder (OCD) and Schizophrenia: Towards New Drug Approach
Comorbidty is common among psychiatric disorders including obsessive-compulsive disorder and schizophrenia with a high rate. Many studies suggested that the disorders may have same etiological bases. In this regard, shared pathways of glutamate, dopaminergic, and serotonin are the known ones. Here, the common significant genes are examined to understand the possible molecular origin of the diso...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2007